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Co‐evolution of the archaeal tRNA‐dependent amidotransferase GatCAB with tRNA Asn
Author(s) -
Namgoong Suk,
Sheppard Kelly,
Sherrer R. Lynn,
Söll Dieter
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.12.033
Subject(s) - transfer rna , glutamine amidotransferase , archaea , biology , biochemistry , enzyme , genetics , chemistry , rna , bacteria , amino acid , glutamine , gene
The important identity elements in tRNA Gln and tRNA Asn for bacterial GatCAB and in tRNA Gln for archaeal GatDE are the D‐loop and the first base pair of the acceptor stem. Here we show that Methanothermobacter thermautotrophicus GatCAB, the archaeal enzyme, is different as it discriminates Asp‐tRNA Asp and Asp‐tRNA Asn by use of U49, the D‐loop and to a lesser extent the variable loop. Since archaea possess the tRNA Gln ‐specific amidotransferase GatDE, the archaeal GatCAB enzyme evolved to recognize different elements in tRNA Asn than those recognized by GatDE or by the bacterial GatCAB enzyme in their tRNA substrates.