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Involvement of phosphatidylinositol‐3‐kinase and ERK pathways in the production of TGF‐β1 by macrophages treated with liposomes composed of phosphatidylserine
Author(s) -
Otsuka Masaki,
Negishi Yoichi,
Aramaki Yukihiko
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.12.032
Subject(s) - phosphatidylinositol , phosphatidylserine , mapk/erk pathway , kinase , microbiology and biotechnology , liposome , chemistry , transforming growth factor , signal transduction , biochemistry , biology , phospholipid , membrane
We explored the involvement of phosphatidylinositol 3‐kinase (PI3K) and ERK pathways in the production of TGF‐β1 by macrophages treated with liposomes composed of phosphatidylserine (PS‐liposomes). PS‐liposomes activated Akt, downstream of the PI3K signal cascade, and ERK which led to the expression of TGF‐β1. PI3K inhibitors, LY294002 and wortmannin, inhibited the activation of Akt and ERK following the treatment with PS‐liposomes. These inhibitors also suppressed the production of TGF‐β1. Furthermore, PS‐liposomes activated macrophages to induce TGF‐β1 expression through PS‐specific receptors. These findings suggested that a PI3K‐ERK signaling pathway via the PS‐receptor is intimately involved in the production of TGF‐β1 which regulates macrophage functions.