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Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822‐2
Author(s) -
Zhang Shuo,
Sakuradani Eiji,
Ito Kuni,
Shimizu Sakayu
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.12.031
Subject(s) - fatty acid desaturase , biochemistry , linoleic acid , polyunsaturated fatty acid , fatty acid , chemistry , stearoyl coa desaturase , yeast , linolenic acid , gene , phosphofructokinase 2 , biology , gene expression
A new gene encoding a Δ12 fatty acid desaturase‐related protein was cloned from a multicellular basidiomycete Coprinus cinereus TD#822‐2. The 1326 bp full‐length gene, designated as Cop‐odeA , codes for a putative protein of 442 amino acids with a MW of 49 224. The Cop‐odeA yeast transformant accumulated four new fatty acids identified as 9,12‐hexadecadienoic acid, 9,12,15‐hexadecatrienoic acid, linoleic acid, and α‐linolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively. The Cop‐odeA protein was confirmed to be a novel bifunctional fatty acid desaturase with both high Δ12 desaturase activity and unusual Δ15 desaturase activity.
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