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RBMX is a novel hepatic transcriptional regulator of SREBP‐1c gene response to high‐fructose diet
Author(s) -
Takemoto Tadashi,
Nishio Yoshihiko,
Sekine Osamu,
Ikeuchi Chikako,
Nagai Yoshio,
Maeno Yasuhiro,
Maegawa Hiroshi,
Kimura Hiroshi,
Kashiwagi Atsunori
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.12.014
Subject(s) - sterol regulatory element binding protein , fructose , biology , transcription factor , gene , electrophoretic mobility shift assay , transcription (linguistics) , microbiology and biotechnology , carbohydrate responsive element binding protein , promoter , gene expression , biochemistry , linguistics , philosophy
In rodents a high‐fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)‐binding protein‐1c (SREBP‐1c) promoter region plays a key role for the response to high‐fructose diet. Here, using MALDI‐TOF MASS technique, we identified an X‐chromosome‐linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti‐RBMX antibody displaced the bands induced by fructose‐feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP‐1c promoter activity. RBMX may control SREBP‐1c expression in mouse liver in response to high‐fructose diet.