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Identification of proteins whose expression is up‐ or down‐regulated in the mushroom bodies in the honeybee brain using proteomics
Author(s) -
Uno Yuko,
Fujiyuki Tomoko,
Morioka Mizue,
Takeuchi Hideaki,
Kubo Takeo
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.12.004
Subject(s) - proteomics , biology , gene expression , proteome , microbiology and biotechnology , two dimensional gel electrophoresis , gel electrophoresis , mushroom bodies , tandem mass spectrometry , mushroom , gene , biochemistry , mass spectrometry , chemistry , chromatography , food science , drosophila melanogaster
To identify protein(s) with different expression patterns in the mushroom bodies (MBs) in the honeybee brain, we compared the protein profiles of MBs and optic lobes (OLs) using proteomics. Two‐dimensional gel electrophoresis revealed that five and three spots were selectively expressed in the MBs or OLs, respectively. Liquid chromatography tandem mass spectrometry analysis identified juvenile hormone diol kinase and glyceraldehyde‐3‐phosphate dehydrogenase as MB‐ and OL‐selective proteins, respectively. In situ hybridization revealed that jhdk expression was upregulated in MB neuron subsets, whereas gapdh expression was downregulated, indicating that MBs have a distinct gene and protein expression profile in the honeybee brain.