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The S. cerevisiae Yap1 and Yap2 transcription factors share a common cadmium‐sensing domain
Author(s) -
Azevedo Dulce,
Nascimento Liliana,
Labarre Jean,
Toledano Michel B.,
Rodrigues-Pousada Claudina
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.083
Subject(s) - yap1 , c terminus , homologous chromosome , transcription factor , chemistry , homology (biology) , cadmium , function (biology) , microbiology and biotechnology , biology , biochemistry , genetics , gene , amino acid , organic chemistry
Towards elucidating the function of Yap2, which remains unclear, we have taken advantage of the C‐terminal homology between Yap1 and Yap2. Swapping domains experiments show that the Yap2 C‐terminal domain functionally substitutes for the homologous Yap1 domain in the response to Cd, but not to H 2 O 2 . We conclude that specificity determinants of the Cd response are encoded within both Yap1 and Yap2 C‐terminus, whereas those required for H 2 O 2 response are only present in the Yap1 C‐terminus. Furthermore, our results identify FRM2 as Cd‐responsive Yap2 target and indicate a possible role of this protein in regulating a metal stress response.