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The plant‐specific VIMAG domain of Glutamine Dumper1 is necessary for the function of the protein in arabidopsis
Author(s) -
Pratelli Réjane,
Pilot Guillaume
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.064
Subject(s) - arabidopsis , glutamine , mutant , function (biology) , arabidopsis thaliana , mutation , arginine , biochemistry , amino acid , protein domain , gene , phenotype , glycine , biology , microbiology and biotechnology , chemistry , genetics
The over‐expression of the arabidopsis GLUTAMINE DUMPER1 gene (GDU1) leads to increased amino acid content and transport. In a screening for mutations suppressing this phenotype, a mutant was isolated. The mutation leads to a glycine to arginine substitution in one of the two conserved domains of the protein, the VIMAG domain. More detailed structure function relationship analyses showed that the presence of this domain and the membrane localisation are both necessary for the function of the GDU1 protein. These results shed light on the function of the GDU1 protein whose family is specific to plants.