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hnRNP‐U enhances the expression of specific genes by stabilizing mRNA
Author(s) -
Yugami Masato,
Kabe Yasuaki,
Yamaguchi Yuki,
Wada Tadashi,
Handa Hiroshi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.062
Subject(s) - heterogeneous nuclear ribonucleoprotein , messenger rna , heterogeneous ribonucleoprotein particle , untranslated region , gene expression , three prime untranslated region , ribonucleoprotein , rna binding protein , gene , microbiology and biotechnology , biology , regulation of gene expression , au rich element , nuclear matrix , chemistry , genetics , rna , chromatin
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre‐mRNA processing. hnRNP‐U, also termed scaffold attachment factor A (SAF‐A), binds to pre‐mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP‐U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP‐U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP‐U enhances the expression of specific genes by regulating mRNA stability.