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Molecular characterization of a glycerophosphoinositol transporter in mammalian cells
Author(s) -
Mariggiò Stefania,
Iurisci Cristiano,
Sebastià Jordi,
Patton-Vogt Jana,
Corda Daniela
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.039
Subject(s) - transporter , characterization (materials science) , chemistry , computational biology , biology , biochemistry , gene , nanotechnology , materials science
The glycerophosphoinositols are ubiquitous phosphoinositide metabolites involved in the control of several cell functions. They exert their actions both intracellularly and by rapidly equilibrating across the plasma membrane when added to cells, implying the existence of a transporter for their membrane permeation. Such a transporter, GIT1 , has been cloned in yeast. By PSI‐BLAST analysis, we have identified the Glut2 transporter as a human‐genome candidate ortholog of GIT1 . This was supported directly through the use of inhibitors, siRNAs and competition studies of specific uptake of GroPIns in HeLa cells over‐expressing human Glut2. These data identify Glut2 as a GroPIns transporter in mammals, and define a physiologically relevant cell‐permeation mechanism.