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Augmentation of 11β‐hydroxysteroid dehydrogenase type 1 in LPS‐activated J774.1 macrophages – Role of 11β‐HSD1 in pro‐inflammatory properties in macrophages
Author(s) -
Ishii Takako,
Masuzaki Hiroaki,
Tanaka Tomohiro,
Arai Naoki,
Yasue Shintaro,
Kobayashi Nozomi,
Tomita Tsutomu,
Noguchi Michio,
Fujikura Junji,
Ebihara Ken,
Hosoda Kiminori,
Nakao Kazuwa
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.032
Subject(s) - adipose tissue , adipose tissue macrophages , inflammation , tumor necrosis factor alpha , lipopolysaccharide , macrophage , secretion , infiltration (hvac) , monocyte , microbiology and biotechnology , cell culture , chemistry , endocrinology , medicine , biology , immunology , biochemistry , white adipose tissue , in vitro , materials science , composite material , genetics
Macrophage infiltration in obese adipose tissue provokes local inflammation and insulin resistance. Evidence has accumulated that activation of 11β‐HSD1 in adipocytes is critically involved in dysfunction of adipose tissue. However, the potential role of 11β‐HSD1 in macrophages still remains unclear. We here demonstrate that a murine macrophage cell line, J774.1 cells expressed 11β‐HSD1 mRNA and reductase activity, both of which were augmented by lipopolysaccharide (LPS)‐induced cell activation. Three kinds of pharmacological inhibition of 11β‐HSD1 in LPS‐treated macrophages significantly suppressed the expression and secretion of interleukin 1β, tumor necrosis factor α or monocyte chemoattractant protein 1, thereby highlighting a novel role of 11β‐HSD1 in pro‐inflammatory properties of activated macrophages.