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Time‐resolved responses to chemoattractant, characteristic of the front and tail of Dictyostelium cells
Author(s) -
Etzrodt Martin,
Ishikawa Hellen C.F.,
Dalous Jeremie,
Müller-Taubenberger Annette,
Bretschneider Till,
Gerisch Günther
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.031
Subject(s) - chemotaxis , dictyostelium , myosin , actin , front (military) , microbiology and biotechnology , polymerization , biology , biophysics , dictyostelium discoideum , chemistry , biochemistry , physics , polymer , gene , receptor , organic chemistry , meteorology
In a gradient of chemoattractant, Dictyostelium cells are orientated with their front directed toward the source and their tail pointing into the opposite direction. The front region is specified by the polymerization of actin and the tail by the recruitment of filamentous myosin‐II. We have dissected these front and tail responses by exposing cells to an upshift of cyclic AMP. A sharp rise and fall of polymerized actin within 10 s is accompanied by the recruitment of proteins involved in turning actin polymerization on or off. The cortical accumulation of myosin‐II starts when the front response has declined, supporting the concept of divergent signal transmission and adaptation pathways.