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The cell membrane‐shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide
Author(s) -
Nishida Takanori,
Orikasa Yoshitake,
Watanabe Kazuo,
Okuyama Hidetoshi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.030
Subject(s) - eicosapentaenoic acid , hydrogen peroxide , catalase , escherichia coli , chemistry , biochemistry , intracellular , bacteria , protein carbonylation , oxidative stress , fatty acid , food science , microbiology and biotechnology , biology , polyunsaturated fatty acid , oxidative damage , genetics , gene
The colony‐forming ability of catalase‐deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3 mM hydrogen peroxide (H 2 O 2 ) under non‐growth conditions. H 2 O 2 ‐induced protein carbonylation was enhanced in cells lacking EPA. The amount of fatty acids was decreased more significantly for cells lacking EPA than for those producing EPA. Much lower intracellular concentrations of H 2 O 2 were detected for cells with EPA than those lacking EPA. These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H 2 O 2 .