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Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway
Author(s) -
Yonezawa Tomo,
Haga Satoshi,
Kobayashi Yosuke,
Takahashi Tatsuyuki,
Obara Yoshiaki
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.014
Subject(s) - mammary gland , forskolin , endocrinology , western blot , medicine , messenger rna , biology , cytosol , epithelium , chemistry , microbiology and biotechnology , cancer , breast cancer , gene , biochemistry , enzyme , genetics , stimulation
Visfatin was originally identified as a growth factor for immature B cells, and recently demonstrated to bind insulin receptor. Visfatin mRNA and protein were detected by RT‐PCR and Western blot analysis in cloned bovine mammary epithelial cells, lactating bovine mammary gland and human breast cancer cell line, MCF‐7. Immunocytochemical staining localized the visfatin protein in the cytosol and nucleus of both cells. Quantitative‐RT‐PCR analysis revealed that the expression of the visfatin mRNA was significantly elevated when treated with forskolin (500 μM), isopreterenol (1–10 μM) and dibutyric cyclic AMP (1 mM) for 24 h, and significantly reduced when treated with insulin (5–50 ng/ml) and dexsamethasone (0.5–250 nM) for 24 h. These results indicate that mammary epithelial cells express the visfatin protein and secrete them into the milk.