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A promoter switch that can rescue a plant sigma factor mutant
Author(s) -
Schweer Jennifer,
Loschelder Heike,
Link Gerhard
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.010
Subject(s) - sigma factor , rna polymerase , operon , mutant , biology , specificity factor , gene , genetics , plastid , promoter , microbiology and biotechnology , rna , sigma , gene expression , chloroplast , physics , quantum mechanics
Chloroplasts sigma factors act in concert with PEP, the bacterial‐type plastid RNA polymerase. Using a sigma knockout line from Arabidopsis thaliana , we investigated mutant‐specific changes in plastid gene expression at RNA level. One characteristic feature was the appearance of a long transcript that spans the atpB‐E operon and extends considerably into the far‐upstream region of atpB . This region reveals a cluster of typical promoter elements for NEP, the second (phage‐type) plastid RNA polymerase. The NEP promoter cluster can help maintain RNA synthesis in situations where no functional sigma factor is available for PEP.