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NMR investigation of DNA primer–template models: Structural insights into dislocation mutagenesis in DNA replication
Author(s) -
Chi Lai Man,
Lam Sik Lok
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.11.001
Subject(s) - primer (cosmetics) , template , primer extension , primase , dna replication , oligonucleotide , dna , dna polymerase , biology , nucleotide , polymerase , mutagenesis , dna clamp , chemistry , computational biology , polymerase chain reaction , genetics , mutation , base sequence , nanotechnology , reverse transcriptase , gene , materials science , organic chemistry
Slipped frameshift intermediates can occur when DNA polymerase slows or stalls at sites of DNA lesions. However, this phenomenon is much less common when unmodified DNA is replicated. In order to study the effect of templating bases on the alignment of primer–templates, NMR structural investigation has been performed on primer–template oligonucleotide models which mimic the situation that dNTP has just been incorporated opposite template. NMR evidence reveals the occurrence of misalignment when dGTP is incorporated opposite template T with a downstream nucleotide C. Depending on the template sequence, further extension of the primer can lead to realignment.