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Casein kinase 1 delta (CK1δ) interacts with the SNARE associated protein snapin
Author(s) -
Wolff Sonja,
Stöter Martin,
Giamas Georgios,
Piesche Matthias,
Henne-Bruns Doris,
Banting George,
Knippschild Uwe
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.10.068
Subject(s) - casein kinase 1 , immunoprecipitation , biology , casein kinase 2 , phosphorylation , biochemistry , microbiology and biotechnology , protein kinase a , gene , mitogen activated protein kinase kinase
In this study we identified snapin as an interaction partner of the CK1 isoform delta (CK1δ) in the yeast two‐hybrid system and localized the interacting domains of both proteins. The interaction of CK1δ with snapin was confirmed by co‐immunoprecipitation. Snapin was phosphorylated by CK1δ in vitro. Both proteins localized in close proximity in the perinuclear region, wherein snapin was found to associate with membranes of the Golgi apparatus. The identification of snapin as a new substrate of CK1δ points towards a possible function for CK1δ in modulating snapin specific functions.