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Differential uptake of photosynthetic and non‐photosynthetic proteins by pea root plastids
Author(s) -
Yan Xianxi,
Khan Sultan,
Hase Toshiharu,
Emes Michael J.,
Bowsher Caroline G.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.10.057
Subject(s) - ferredoxin , plastid , chloroplast , ferredoxin thioredoxin reductase , transit peptide , photosynthesis , ferredoxin—nadp(+) reductase , rubisco , biology , biochemistry , botany , enzyme , glutathione , thioredoxin reductase , gene
The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP + ‐oxidoreductase ( p FNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non‐photosynthetic ferredoxin III and heterotrophic FNR ( h FNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus.