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Reversible skeletal neuromuscular paralysis induced by different lysophospholipids
Author(s) -
Caccin Paola,
Rigoni Michela,
Bisceglie Alessandra,
Rossetto Ornella,
Montecucco Cesare
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.10.039
Subject(s) - paralysis , chemistry , periodic paralysis , medicine , neuroscience , biology , surgery
Lysophosphatidylcholine rapidly paralyses the neuromuscular junction (NMJ), similarly to snake phospholipase A2 neurotoxins, implicating a lipid hemifusion‐pore transition in neuroexocytosis. The mode and kinetics of NMJ paralysis of different lysophospholipids (lysoPLs) in high or low [Mg 2+ ] was investigated. The following order of potency was found: lysophosphatidylcholine > lysophosphatidylethanolamine > lysophosphatidic acid > lysophosphatidylserine > lysophosphatidylglycerol. The latter two lysoPLs closely mimic the profile of paralysis caused by the toxins in high [Mg 2+ ]. This paralysis is fully reversed by albumin washing. These findings provide novel insights on the mode of action of snake neurotoxins and qualify lysoPLs as novel agents to study neuroexocytosis.