Premium
Specific short hairpin RNA‐mediated inhibition of viral DNA packaging of human cytomegalovirus
Author(s) -
Dittmer Alexandra,
Bogner Elke
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.10.011
Subject(s) - small hairpin rna , transduction (biophysics) , human cytomegalovirus , rna interference , rna , virology , dna , microbiology and biotechnology , biology , messenger rna , cell culture , viral vector , cytomegalovirus , virus , chemistry , gene , herpesviridae , genetics , viral disease , biochemistry , recombinant dna
To clearly demonstrate the role of the human cytomegalovirus (HCMV) portal protein pUL104 RNA interference was applied. Expressing cell lines were constructed by transduction of shRNAs via the infection with retroviral vectors. After infection of these cells with HCMV AD169 the expression of pUL104 was reduced up to 80% for shRNA S1 and 54% for shRNA S2 at late times of infection compared to controls. In addition, the inhibitory effect was corresponding with a decrease in viral mRNA and plaque formations. Electron microscopic analysis demonstrated that infection of cells expressing pUL104‐specific shRNAs resulted in the inhibition of formation of replicative particles.