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The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I
Author(s) -
Seo Byoung Boo,
Marella Mathieu,
Yagi Takao,
Matsuno-Yagi Akemi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.10.008
Subject(s) - reactive oxygen species , rotenone , oxidative stress , nadh dehydrogenase , mitochondrion , lipid peroxidation , biochemistry , protein subunit , oxidative phosphorylation , biology , electron transport complex i , enzyme , mitochondrial dna , saccharomyces cerevisiae , microbiology and biotechnology , chemistry , respiratory chain , gene
Using rat dopaminergic and human neuroblastoma cell lines transduced with the NDI1 gene encoding the internal NADH dehydrogenase (Ndi1) from Saccharomyces cerevisiae , we investigated reactive oxygen species (ROS) generation caused by complex I inhibition. Incubation of non‐transduced cells with rotenone elicited oxidative damage to mitochondrial DNA as well as lipid peroxidation. In contrast, oxidative stress was significantly decreased when the cells were transduced with NDI1 . Furthermore, mitochondria from the NDI1 ‐transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors. We conclude that the Ndi1 enzyme is able to suppress ROS overproduction from defective complex I.