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Characterization of DIDS‐sensitive ATP accumulation in brain synaptic vesicles
Author(s) -
Zalk Ran,
Shoshan-Barmatz Varda
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.055
Subject(s) - synaptic vesicle , dids , vesicle , chemistry , biophysics , nucleotide , excitatory postsynaptic potential , neurotransmitter , biochemistry , biology , membrane , receptor , gene
ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a ΔμH + ‐independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg 2+ or by pre‐incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.