Premium
Caspase‐mediated changes in Sir2α during apoptosis
Author(s) -
Ohsawa Shizue,
Miura Masayuki
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.051
Subject(s) - microbiology and biotechnology , caenorhabditis elegans , apoptosis , caspase , chromatin , biology , histone , regulator , histone deacetylase , caspase 2 , acetylation , cytoplasm , mutant , poly adp ribose polymerase , programmed cell death , genetics , gene , polymerase
Silent information regulator 2 (Sir2) is an NAD + ‐dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans . There is growing evidence that its mammalian homologue Sir2α protects cells from stress‐induced apoptosis. We report here that mammalian Sir2α was directly cleaved by both initiator and executioner caspases, and relocated from the nucleus to the cytoplasm in apoptotic cells. These alterations of Sir2α were largely inhibited by a caspase‐9 dominant‐negative mutant or Bcl‐xL. Our results indicate that Sir2α undergoes dynamic changes in caspase‐dependent manner during apoptosis.