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Premium Characterization of novel imidazole derivative, JM‐8686, a potent inhibitor of allene oxide synthase
Author(s)
Oh Keimei,
Asami Tadao,
Matsui Kenji,
Howe Gregg A.,
Murofushi Noboru
Publication year2006
Publication title
febs letters
Resource typeJournals
PublisherElsevier BV
The inhibitory properties of a first synthetic jasmonic acid biosynthesis inhibitor, JM‐8686, were investigated. Steady‐state kinetic analysis indicates that the compound is a competitive inhibitor of allene oxide synthase (AOS) with a K i value of approximate 0.62 ± 0.15 μM. Dialysis experiment indicates that AOS inactivation by JM‐8686 is reversible. The optical difference spectroscopy analysis of JM‐8686 and AOS interaction indicates that JM‐8686 induced type II binding spectra with a K d value of approximate 1.6 ± 0.2 μM, suggesting that JM‐8686 binds to the prosthetic heme iron of AOS. Comparison of the inhibitory potency of the compound against HPL (CYP74B) from tomato revealed that JM‐8686 was a highly selective inhibitor for AOS.
Subject(s)allene , atp synthase , biochemistry , biology , biosynthesis , catalysis , chemistry , derivative (finance) , economics , enzyme , financial economics , inhibitory postsynaptic potential , neuroscience , nitric oxide synthase , stereochemistry
Language(s)English
SCImago Journal Rank1.593
H-Index257
eISSN1873-3468
pISSN0014-5793
DOI10.1016/j.febslet.2006.09.044

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