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Endothelin‐1 inhibits adipogenesis: Role of phosphorylation of Akt and ERK1/2
Author(s) -
Bhattacharya Indranil,
Ullrich Axel
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.032
Subject(s) - adipogenesis , phosphorylation , protein kinase b , epidermal growth factor , medicine , endocrinology , chemistry , stimulation , microbiology and biotechnology , adipocyte , endothelin 1 , growth factor , biology , biochemistry , adipose tissue , receptor
In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin‐1 (ET‐1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3‐L1 cells. ET‐1 stimulation caused an anti‐adipogenic response and this effect was potentiated upon treatment with EGF. Co‐treatment with EGF and ET‐1 blocked the expression of C/EBPα and PPARγ, the adipogenic markers. The inhibition of adipogenesis was preceded by a biphasic (early and late) attenuation of Akt phosphorylation. We suggest that treatment with ET‐1 and EGF together induce a more potent anti‐adipogenic response, involving increased Erk1/2 phosphorylation and biphasic attenuation of Akt phosphorylation.