Premium
Occurrence of A‐kinase anchor protein and associated cAMP‐dependent protein kinase in the inner compartment of mammalian mitochondria
Author(s) -
Sardanelli Anna Maria,
Signorile Anna,
Nuzzi Rosanna,
Rasmo Domenico De,
Technikova-Dobrova Zuzana,
Drahota Zdenek,
Occhiello Antonella,
Pica Alessandra,
Papa Sergio
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.020
Subject(s) - compartment (ship) , protein kinase a , microbiology and biotechnology , cgmp dependent protein kinase , mitochondrion , chemistry , kinase , mitogen activated protein kinase kinase , ask1 , biochemistry , biology , oceanography , geology
Evidence showing the existence in the inner compartment of rat‐heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C‐PKA, R‐PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R‐PKA by 32 P‐phosphorylated C‐PKA and of AKAP by 32 P‐phosphorylated R‐PKA and electron microscopy of mitochondria exposed to gold‐conjugated AKAP121 antibody.