Observation of single‐stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy | Zendy

Adamcik Jozef | Zendy; Klinov Dmitry V. | Zendy; Witz Guillaume | Zendy; Sekatskii Sergey K. | Zendy; Dietler Giovanni | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Observation of single‐stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy

Author(s) -

Adamcik Jozef,

Klinov Dmitry V.,

Witz Guillaume,

Sekatskii Sergey K.,

Dietler Giovanni

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.09.017

Subject(s) - mica , highly oriented pyrolytic graphite , pyrolytic carbon , graphite , atomic force microscopy , intramolecular force , molecule , nanotechnology , materials science , dna , adsorption , crystallography , microscopy , chemical physics , chemistry , stereochemistry , organic chemistry , composite material , optics , physics , pyrolysis , biochemistry

Atomic force microscopy was used to image single‐stranded DNA (ssDNA) adsorbed on mica modified by Mg 2+ , by 3‐aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base‐pairing, thus this method could be important for studying certain processes involving ssDNA in more details.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research