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Observation of single‐stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy
Author(s) -
Adamcik Jozef,
Klinov Dmitry V.,
Witz Guillaume,
Sekatskii Sergey K.,
Dietler Giovanni
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.017
Subject(s) - mica , pyrolytic carbon , highly oriented pyrolytic graphite , atomic force microscopy , graphite , intramolecular force , molecule , materials science , adsorption , crystallography , nanotechnology , dna , microscopy , conductive atomic force microscopy , chemical physics , force spectroscopy , chemistry , chemical engineering , stereochemistry , composite material , organic chemistry , optics , physics , pyrolysis , biochemistry , engineering
Atomic force microscopy was used to image single‐stranded DNA (ssDNA) adsorbed on mica modified by Mg 2+ , by 3‐aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base‐pairing, thus this method could be important for studying certain processes involving ssDNA in more details.