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Bimodal actions of reactive oxygen species in the differentiation and bone‐resorbing functions of osteoclasts
Author(s) -
Kim Hyunsoo,
Kim Ick Young,
Lee Soo Young,
Jeong Daewon
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.015
Subject(s) - osteoclast , glutathione , bone resorption , oxidizing agent , resorption , redox , chemistry , reactive oxygen species , endocrinology , microbiology and biotechnology , glutathione disulfide , biochemistry , medicine , enzyme , biology , in vitro , inorganic chemistry , organic chemistry
In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ‐glutamylcysteinyl synthetase activity, a glutathione synthesis rate‐limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.