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Differential protein distributions define two sub‐compartments of the mitochondrial inner membrane in yeast
Author(s) -
Wurm Christian A.,
Jakobs Stefan
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.012
Subject(s) - translocase of the inner membrane , inner membrane , mitochondrial carrier , inner mitochondrial membrane , microbiology and biotechnology , atp–adp translocase , mitochondrion , translocase of the outer membrane , mitochondrial membrane transport protein , mitochondrial fusion , biology , chemistry , biochemistry , bacterial outer membrane , mitochondrial dna , gene , escherichia coli
The mitochondrial inner membrane exhibits a complex topology. Its infolds, the cristae membranes, are contiguous with the inner boundary membrane (IBM), which runs parallel to the outer membrane. Using live cells co‐expressing functional fluorescent fusion proteins, we report on the distribution of inner membrane proteins in budding yeast. To this end we introduce the enlarged mitochondria of Δ mdm10 , Δ mdm31 , Δ mdm32 , and Δ mmm1 cells as a versatile model system to study sub‐mitochondrial protein localizations. Proteins of the F 1 F 0 ATP synthase and of the respiratory chain complexes III and IV were visualized in the cristae‐containing interior of the mitochondria. In contrast, proteins of the TIM23 complex and of the presequence translocase‐associated motor were strongly enriched at the IBM. The different protein distributions shown here demonstrate that the cristae membranes and the IBM are functionally distinct sub‐compartments.