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WFS1 protein modulates the free Ca 2+ concentration in the endoplasmic reticulum
Author(s) -
Takei Daisuke,
Ishihara Hisamitsu,
Yamaguchi Suguru,
Yamada Takahiro,
Tamura Akira,
Katagiri Hideki,
Maruyama Yoshio,
Oka Yoshitomo
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.09.007
Subject(s) - endoplasmic reticulum , aequorin , wolfram syndrome , chemistry , microbiology and biotechnology , gene knockdown , homeostasis , hek 293 cells , biology , intracellular , biochemistry , apoptosis , gene
The WFS1 gene, encoding an endoplasmic reticulum (ER) membrane glycoprotein, is mutated in Wolfram syndrome characterized by diabetes mellitus and optic atrophy. Herein, Ca 2+ dynamics were examined in WFS1‐knockdown and ‐overexpressing HEK293 cells. Studies using ER‐targeted Ca 2+ ‐sensitive photoprotein aequorin demonstrated WFS1 protein to positively modulate ER Ca 2+ levels by increasing the rate of Ca 2+ uptake. Furthermore, Ca 2+ imaging with Fura‐2 showed the magnitude of the store‐operated Ca 2+ entry to parallel WFS1 expression levels. These data indicate that WFS1 protein participates in the regulation of cellular Ca 2+ homeostasis, at least partly, by modulating the filling state of the ER Ca 2+ store.