WFS1 protein modulates the free Ca 2+  concentration in the endoplasmic reticulum | Zendy

Takei Daisuke | Zendy; Ishihara Hisamitsu | Zendy; Yamaguchi Suguru | Zendy; Yamada Takahiro | Zendy; Tamura Akira | Zendy; Katagiri Hideki | Zendy; Maruyama Yoshio | Zendy; Oka Yoshitomo | Zendy

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WFS1 protein modulates the free Ca 2+ concentration in the endoplasmic reticulum

Author(s) -

Takei Daisuke,

Ishihara Hisamitsu,

Yamaguchi Suguru,

Yamada Takahiro,

Tamura Akira,

Katagiri Hideki,

Maruyama Yoshio,

Oka Yoshitomo

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.09.007

Subject(s) - endoplasmic reticulum , chemistry , stim1 , microbiology and biotechnology , biophysics , biochemistry , biology

The WFS1 gene, encoding an endoplasmic reticulum (ER) membrane glycoprotein, is mutated in Wolfram syndrome characterized by diabetes mellitus and optic atrophy. Herein, Ca 2+ dynamics were examined in WFS1‐knockdown and ‐overexpressing HEK293 cells. Studies using ER‐targeted Ca 2+ ‐sensitive photoprotein aequorin demonstrated WFS1 protein to positively modulate ER Ca 2+ levels by increasing the rate of Ca 2+ uptake. Furthermore, Ca 2+ imaging with Fura‐2 showed the magnitude of the store‐operated Ca 2+ entry to parallel WFS1 expression levels. These data indicate that WFS1 protein participates in the regulation of cellular Ca 2+ homeostasis, at least partly, by modulating the filling state of the ER Ca 2+ store.

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