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Shedding of procoagulant microparticles from unstimulated platelets by integrin‐mediated destabilization of actin cytoskeleton
Author(s) -
Cauwenberghs Sandra,
Feijge Marion A.H.,
Harper Alan G.S.,
Sage Stewart O.,
Curvers Joyce,
Heemskerk Johan W.M.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.082
Subject(s) - platelet , microparticle , coagulation , chemistry , microbiology and biotechnology , platelet activation , cytoskeleton , actin cytoskeleton , hemostasis , fibrinogen , integrin , actin , biophysics , biochemistry , immunology , receptor , biology , medicine , cell , astrobiology
Platelet activation by potent, Ca 2+ ‐mobilizing agonists results in shedding of microparticles that are active in coagulation. Here we show that platelets under storage produce procoagulant microparticles in the absence of agonist. Microparticle formation by resting platelets results from αIIbβ3 signaling to destabilization of the actin cytoskeleton in the absence of calpain activation. Integrin‐mediated spreading of platelets over fibrinogen similarly results in microparticle formation. After transfusion of stored platelet preparations to thrombocytopenic patients, the microparticles contribute to coagulant activity in vivo.