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The incorporation of nucleoside analogs by human immunodeficiency virus type 1 reverse transcriptase decreases in the presence of polyamines
Author(s) -
Bakhanashvili Mary,
Rahav Galia
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.074
Subject(s) - reverse transcriptase , spermidine , spermine , nucleoside , dna , biology , putrescine , rna , nucleoside reverse transcriptase inhibitor , rna directed dna polymerase , dna synthesis , nucleic acid , biochemistry , polyamine , microbiology and biotechnology , nucleoside analogue , enzyme , gene
Nucleoside analogs (NAs) are an important class of anti‐retroviral compounds used against human immunodeficiency virus (HIV). We have analyzed the potential effect of polyamines on the incorporation of NAs during DNA synthesis by HIV type‐1 (HIV‐1) reverse transcriptase (RT). The polyamines exert the ability to decrease the incorporation of various dideoxynucleoside triphosphates (ddATP, ddTTP or ddCTP) with both RNA/DNA and DNA/DNA substrates in the following order: spermine > spermidine > putrescine. The reduction is a sequence‐independent effect, taking place at different sequence context. The results suggest that polyamines might affect the inhibition of reverse transcription by nucleoside analogs HIV‐1 RT directed.