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Geranylgeranyl‐pyrophosphate (GGPP) synthase is down‐regulated during differentiation of osteoblastic cell line MC3T3‐E1
Author(s) -
Yoshida Tomohiko,
Asanuma Mie,
Grossmann Laura,
Fuse Masami,
Shibata Takahisa,
Yonekawa Taeko,
Tanaka Tomoaki,
Ueno Koichi,
Yasuda Tatsuji,
Saito Yasushi,
Tatsuno Ichiro
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.060
Subject(s) - geranylgeranyl pyrophosphate , pyrophosphate , farnesyl pyrophosphate , cell culture , microbiology and biotechnology , chemistry , atp synthase , biology , biochemistry , enzyme , prenylation , genetics
Isoprenylation of geranylgeranyl‐pyrophosphate (GGPP) is critical for activation of small GTPases. We examined the roles of GGPP synthase (GGPPS) during the differentiation induced by the cell‐to‐cell contact in osteoblastic cell line MC3T3‐E1 cells. We found that (1) both mRNA and protein expression of GGPPS was reduced with decrement of its activity during the differentiation, (2) GGOH, which is converted to GGPP in the cells, inhibited differentiation. These results suggest that the decrement of GGPP is critical for the cell‐to‐cell contact‐induced differentiation, in which the down‐regulation of GGPPS might be involved.