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Diffusion of the mu opioid receptor at the surface of human neuroblastoma SH‐SY5Y cells is restricted to permeable domains
Author(s) -
Saulière Aude,
Gaibelet Gérald,
Millot Claire,
Mazères Serge,
Lopez André,
Salomé Laurence
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.056
Subject(s) - sh sy5y , receptor , fluorescence recovery after photobleaching , neuroblastoma , biophysics , photobleaching , diffusion , chemistry , fluorescence , membrane , biology , physics , biochemistry , optics , cell culture , thermodynamics , genetics
Previous single‐molecule studies have shown a long‐term diffusion superimposed to a short‐term confinement of the human mu opioid (hMOP) receptors at the surface of heterologous cells. However, additional ensemble average measurements are required to reach a complete understanding of the undergoing process. Here, we analyse, by fluorescence recovery after photobleaching measurements, the lateral diffusion of fully functional T7‐EGFP‐hMOP receptors in neuroblastoma SH‐SY5Y cells naturally expressing a low level of the wild‐type receptor. Experiments carried out at variable observation radii demonstrate the restriction of the receptors diffusion to sub‐micrometer sized domains. Furthermore, consistently with the long‐term single‐molecule data, the domains are found permeable.