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Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures: Preparation, bulk and single molecule spectroscopy
Author(s) -
Brotosudarmo T.H.P.,
Hofmann E.,
Hiller R.G.,
Wörmke S.,
Mackowski S.,
Zumbusch A.,
Bräuchle C.,
Scheer H.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.049
Subject(s) - peridinin , pigment , chlorophyll , chlorophyll a , chemistry , spectroscopy , chlorophyll b , fluorescence , molecule , photochemistry , photosynthetic pigment , fluorescence spectroscopy , absorption (acoustics) , photosynthesis , crystallography , materials science , biochemistry , fucoxanthin , organic chemistry , physics , composite material , quantum mechanics
Reconstitution of the 16 kDa N‐terminal domain of the peridinin–chlorophyll–protein, N‐PCP, with mixtures of chlorophyll a (Chl a ) and Chl b , resulted in 32 kDa complexes containing two pigment clusters, each bound to one N‐PCP. Besides homo‐chlorophyllous complexes, hetero‐chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a . Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo‐ and hetero‐chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.