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Activation of diacylglycerol O ‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes
Author(s) -
Hirata Takashi,
Unoki Hiroyuki,
Bujo Hideaki,
Ueno Koichi,
Saito Yasushi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.047
Subject(s) - gene knockdown , tumor necrosis factor alpha , diacylglycerol kinase , 3t3 l1 , gene expression , downregulation and upregulation , adipocyte , endocrinology , chemistry , alpha (finance) , medicine , triglyceride , lipid droplet , biology , gene , biochemistry , adipose tissue , enzyme , cholesterol , protein kinase c , construct validity , nursing , patient satisfaction
The tumor necrosis factor‐α (TNF‐α) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O ‐acyltransferase (DGAT) in order to clarify the mechanism of TNF‐α expression induced in large adipocytes. 3T3‐L1 cells were cultured in the presence of 5 mM or 25 mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF‐α, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25 mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF‐α expression. Finally, the DGAT1 expression levels were closely related to the TNF‐α level in 3T3‐L1 adipocytes.