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KDC2, a functional homomeric potassium channel expressed during carrot embryogenesis
Author(s) -
Formentin Elide,
Naso Alessia,
Varotto Serena,
Picco Cristiana,
Gambale Franco,
Lo Schiavo Fiorella
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.08.017
Subject(s) - homomeric , somatic embryogenesis , xenopus , daucus carota , potassium channel , microbiology and biotechnology , guard cell , hypocotyl , biology , embryogenesis , embryo , chemistry , botany , protein subunit , biophysics , biochemistry , gene
In Daucus carota , the model system for embryogenesis, it has been demonstrated that potassium and K + selective channels are involved in embryo development. Here, we report the isolation and cloning of a new carrot Shaker ‐like potassium channel, potassium D. carota channel 2 (KDC2), whose expression pattern during somatic embryogenesis proceeds along with the establishment of the polar axes and the settlement of the hypocotyl region. In plants, KDC2 transcript is localized at the shoot level, in the epidermis and guard cells, similarly to its Arabidopsis homolog KAT1 . Electrophysiological assays indicated KDC2 as the first carrot subunit able to form homomeric functional channels in Xenopus oocytes, with properties similar to those of Arabidopsis KAT1.