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Regulation of the rat brain Na + ‐driven Cl ‐ / HCO 3 ‐ exchanger involves protein kinase A and a multiprotein signaling complex
Author(s) -
Lee Yong-Sun,
Ouyang Yi-Bing,
Giffard Rona G.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.075
Subject(s) - multiprotein complex , pdz domain , forskolin , microbiology and biotechnology , cytoskeleton , actin cytoskeleton , intracellular , phosphorylation , actin , signal transduction , protein kinase a , immunoprecipitation , chemistry , sodium–hydrogen antiporter , biology , biochemistry , cell , receptor , gene , organic chemistry , sodium
The Na + ‐drivenCl ‐ / HCO 3 ‐exchanger (NCBE) plays an important role in the regulation of intracellular pH (pH i ). We previously identified two variants of NCBE from rat brain of which the variant with a carboxyterminal PSD‐95/Dlg/ZO‐1 (PDZ) motif (rb2NCBE) colocalized with the actin cytoskeleton. Increased rb2NCBE activity by PKA inhibition and reduction by forskolin and cAMP agonist suggest PKA regulation of NCBE. Disruption of actin filaments also decreased rb2NCBE activity. EBP50 and FLAG‐rb2NCBE were reciprocally co‐immunoprecipitated from rb2NCBE transfected cells. It is concluded that NCBE activity is inhibited by PKA and depends on the integrity of the actin cytoskeleton within a multiprotein complex at the plasma membrane.