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Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti
Author(s) -
Reis Y.,
Cortes H.,
Viseu Melo L.,
Fazendeiro I.,
Leitão A.,
Soares H.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.050
Subject(s) - microtubule , cytoskeleton , microbiology and biotechnology , immunofluorescence , biology , parasite hosting , tubulin , cell , host (biology) , immunology , genetics , antibody , world wide web , computer science
Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoiti undergoes dramatic modifications of shape and surface, as revealed by atomic force microscopy, accompanied by a distinct tubulin labeling on the posterior region. In the host cell, the microtubule cytoskeleton shows a re‐arrangement around the invading parasite suggesting a filamentous interaction with the parasite cytoskeleton during invasion.