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A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis
Author(s) -
Robert Nadia,
Merlot Sylvain,
N’Guyen Vinh,
Boisson-Dernier Aurélien,
Schroeder Julian I.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.047
Subject(s) - arabidopsis , abscisic acid , mutant , arabidopsis thaliana , phosphatase , biology , regulator , mutation , pyruvate dehydrogenase phosphatase , signal transduction , microbiology and biotechnology , function (biology) , phenotype , genetics , phosphorylation , biochemistry , gene , enzyme , pyruvate carboxylase
Protein phosphatases of the 2C family (PP2C) function in the regulation of several signaling pathways from prokaryotes to eukaryotes. In Arabidopsis thaliana , the HAB1 PP2C is a negative regulator of the stress hormone abscisic acid (ABA) signaling. Here, we show that plants expressing a mutant form of HAB1 in which Gly246 was mutated to Asp (G246D) display strong ABA insensitive phenotypes. Our results indicate that the G246D mutation has a hypermorphic rather than a dominant negative effect. The data suggest that this mutation localized in a conserved motif in the PP2C catalytic domain could be used in other PP2Cs to reveal their biological functions.