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Glucuronoyl esterase – Novel carbohydrate esterase produced by Schizophyllum commune
Author(s) -
Špániková Silvia,
Biely Peter
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.033
Subject(s) - schizophyllum commune , esterase , chemistry , biochemistry , hydrolysis , hemicellulose , lignin , carbohydrate , enzyme , organic chemistry
The cellulolytic system of the wood‐rotting fungus Schizophyllum commune contains an esterase that hydrolyzes methyl ester of 4‐ O ‐methyl‐ d ‐glucuronic acid. The enzyme, called glucuronoyl esterase, was purified to electrophoretic homogeneity from a cellulose‐spent culture fluid. Its substrate specificity was examined on a number of substrates of other carbohydrate esterases such as acetylxylan esterase, feruloyl esterase and pectin methylesterase. The glucuronoyl esterase attacks exclusively the esters of MeGlcA. The methyl ester of free or glycosidically linked MeGlcA was not hydrolysed by other carbohydrate esterases. The results suggest that we have discovered a new type of carbohydrate esterase that might be involved in disruption of ester linkages connecting hemicellulose and lignin in plant cell walls.