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Human and mouse hemoglobin association with the transgenic mouse erythrocyte membrane
Author(s) -
Chen Qiuying,
Balazs Tania C.,
Nagel Ronald L.,
Hirsch Rhoda Elison
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.021
Subject(s) - hemoglobin , transgene , genetically modified mouse , membrane , red blood cell , erythrocyte membrane , biology , membrane protein , biochemistry , chemistry , microbiology and biotechnology , gene
Transgenic mouse models of hemoglobinopathies unravel pathophysiological mechanisms; yet the validity of the red blood cell (RBC) model of human hemoglobin (hHb) enveloped by a mouse (m) membrane has been questioned. Isoelectric focusing of hHb and mHb from transgenic mRBC shows a greater association of mHb to the mouse membrane compared to normal hHbA, supporting a species‐specific Hb‐mRBC membrane interaction. Enhanced hmutant Hb (HbE, HbS and HbC)‐mRBC membrane affinities correlates with enhanced membrane lipid peroxidation and parallel those reported in hRBC, lending support to transgenic mRBC as models of hemoglobinopathies. Species‐specific Hb‐membrane interaction may be overridden by Hb charge and conformational alterations.