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Expressed sequence tags from Madagascar periwinkle ( Catharanthus roseus )
Author(s) -
Murata Jun,
Bienzle Dorothee,
Brandle Jim E.,
Sensen Christoph W.,
De Luca Vincenzo
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.020
Subject(s) - catharanthus roseus , botany , expressed sequence tag , sequence (biology) , biology , biochemistry , gene , gene expression
The Madagascar periwinkle ( Catharanthus roseus ) is well known to produce the chemotherapeutic anticancer agents, vinblastine and vincristine. In spite of its importance, no expressed sequence tag (EST) analysis of this plant has been reported. Two cDNA libraries were generated from RNA isolated from the base part of young leaves and from root tips to select 9824 random clones for unidirectional sequencing, to yield 3327 related sequences and 1696 singletons by cluster analysis. Putative functions of 3663 clones were assigned, from 5023 non‐redundant ESTs to establish a resource for transcriptome analysis and gene discovery in this medicinal plant.