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A phosphopantetheinyl transferase gene essential for biosynthesis of n − 3 polyunsaturated fatty acids from Moritella marina strain MP‐1
Author(s) -
Orikasa Yoshitake,
Nishida Takanori,
Hase Akira,
Watanabe Kazuo,
Morita Naoki,
Okuyama Hidetoshi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.07.008
Subject(s) - polyunsaturated fatty acid , docosahexaenoic acid , eicosapentaenoic acid , biosynthesis , biochemistry , biology , escherichia coli , gene , open reading frame , transferase , amino acid , fatty acid , enzyme , peptide sequence
A phosphopantetheinyl transferase (PPTase) gene ( pfaE ), cloned from the docosahexaenoic acid (DHA)‐producing bacterium Moritella marina strain MP‐1, has an open reading frame of 861 bp encoding a 287‐amino acid protein. When the pfaE gene was expressed with pfaA – D , which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC‐2738 in Escherichia coli , the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n − 3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP‐1.