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Conditional gene modification in mouse liver using hydrodynamic delivery of plasmid DNA encoding Cre recombinase
Author(s) -
Zhu Huan Zhang,
Wang Wei,
Feng Deng Min,
Sai Yin,
Xue Jing Lun
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.094
Subject(s) - cre recombinase , microbiology and biotechnology , gene delivery , biology , transgene , gene , transduction (biophysics) , cre lox recombination , recombinase , green fluorescent protein , flow cytometry , site specific recombination , genetically modified mouse , transfection , genetics , recombination , biochemistry
The success of Cre‐mediated conditional gene targeting in liver of mice has until now depended on the generation of Cre recombinase transgenic mice or on viral‐mediated transduction. Here, we sought to establish the feasibility of using hydrodynamic gene delivery of Cre recombinase into liver, using a ROSA26 EGFP mouse. The expression of EGFP and β‐galactosidase was exclusively detected in the liver of mice treated with hydrodynamic gene delivery of Cre recombinase, as assessed with fluorescence microscopy and X‐Gal staining, respectively; Southern blotting also showed that Cre mediated recombination occurred specifically in the liver and not in other organs. The Cre mediated recombination reached about 61% of hepatocytes of mouse after repeated injection, as analyzed by flow cytometry. These results demonstrate that Cre recombinase can be transferred to the liver of mice through a simple hydrodynamic gene‐delivery approach and can mediate efficient recombination in hepatocytes. Thus, hydrodynamic gene delivery of the Cre recombinase provides a valuable approach for Cre‐loxP‐mediated conditional gene modification in the liver of mice.