Premium
Characterization of the last step of lignin biosynthesis in Zinnia elegans suspension cell cultures
Author(s) -
Gabaldón Carlos,
López-Serrano Matías,
Pomar Federico,
Merino Fuencisla,
Cuello Juan,
Pedreño M.A.,
Barceló A. Ros
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.088
Subject(s) - monolignol , lignin , biosynthesis , cell wall , chemistry , biochemistry , suspension culture , coniferyl alcohol , peroxidase , suspension (topology) , cell culture , biology , enzyme , organic chemistry , genetics , mathematics , homotopy , pure mathematics
The last step of lignin biosynthesis in Zinnia elegans suspension cell cultures (SCCs) catalyzed by peroxidase (ZePrx) has been characterized. The k 3 values shown by ZePrx for the three monolignols revealed that sinapyl alcohol was the best substrate, and were proportional to their oxido/reduction potentials, signifying that these reactions are driven exclusively by redox thermodynamic forces. Feeding experiments demonstrate that cell wall lignification in SCCs is controlled by the rate of supply of H 2 O 2 . The results also showed that sites for monolignol β‐ O ‐4 cross‐coupling in cell walls may be saturated, suggesting that the growth of the lineal lignin macromolecule is not infinite.