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cAMP controls oxygen metabolism in mammalian cells
Author(s) -
Piccoli Claudia,
Scacco Salvatore,
Bellomo Francesco,
Signorile Anna,
Iuso Arcangela,
Boffoli Domenico,
Scrima Rosella,
Capitanio Nazzareno,
Papa Sergio
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.085
Subject(s) - reactive oxygen species , protein subunit , microbiology and biotechnology , oxidoreductase , metabolism , chemistry , biochemistry , enzyme activator , electron transport complex i , biology , enzyme , gene
The impact of cAMP on ROS‐balance in human and mammalian cell cultures was studied. cAMP reduced accumulation of ROS induced by serum‐limitation, under conditions in which there was no significant change in the activity of scavenger systems. This effect was associated with cAMP‐dependent activation of the NADH‐ubiquinone oxidoreductase activity of complex I. In fibroblasts from a patient a genetic defect in the 75 kDa FeS‐protein subunit of complex I resulted in inhibition of the activity of the complex and enhanced ROS production, which were reversed by cAMP. A missense genetic defect in the NDUFS4 subunit, putative substrate of PKA, suppressed, on the other hand, the activity of the complex and prevented ROS production.