Premium
CITRX thioredoxin is a putative adaptor protein connecting Cf‐9 and the ACIK1 protein kinase during the Cf‐9/Avr9‐ induced defence response
Author(s) -
Nekrasov Vladimir,
Ludwig Andrea A.,
Jones Jonathan D.G.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.077
Subject(s) - signal transducing adaptor protein , cytoplasm , protein kinase domain , microbiology and biotechnology , kinase , biology , grb2 , chemistry , gene , biochemistry , signal transduction , mutant
Tomato Cf ‐ 9 , a receptor‐like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf‐9‐interacting thioredoxin) was previously identified in a yeast two‐hybrid screen as a protein interacting with the cytoplasmic domain of Cf‐9 and shown to be a negative regulator of the cell death induced after Cf‐9/Avr9 interaction. ACIK1 is a Ser/Thr protein kinase that is specifically required for the Cf ‐ 9 and Cf ‐ 4 dependent defence response in tomato. In this paper we present data suggesting that CITRX may act as an adaptor recruiting the ACIK1 kinase to the cytoplasmic domain of Cf‐9 upon elicitation with the Avr9 peptide. Interestingly, the catalytic activities of both CITRX and ACIK1 are not required for their interaction.