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Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells
Author(s) -
Deshpande Alina,
Hammon Rebecca J.,
Sanders Claire K.,
Graves Steven W.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.070
Subject(s) - microbiology and biotechnology , receptor , chemistry , cellular differentiation , antigen , in vitro , extracellular matrix , cell , biology , immunology , biochemistry , gene
We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined with the function of such cells, this suggests that PA receptors interact with the extracellular matrix and that differentiation increases the number of PA‐specific receptors, which supports previously observed differentiation‐induced LT susceptibility. Our results quantifiably confirm that the generality of PA binding will complicate its use as a tumor targeting agent.