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Transient suppression of PPARγ directed ES cells into an osteoblastic lineage
Author(s) -
Yamashita Akihiro,
Takada Tatsuyuki,
Nemoto Ken-ichi,
Yamamoto Gaku,
Torii Ryuzo
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.057
Subject(s) - downregulation and upregulation , osteocalcin , peroxisome proliferator activated receptor , microbiology and biotechnology , lineage (genetic) , progenitor cell , chemistry , adipogenesis , osteoblast , receptor , cellular differentiation , biology , stem cell , mesenchymal stem cell , alkaline phosphatase , biochemistry , in vitro , gene , enzyme
Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator‐activated receptor γ (PPARγ) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARγ without the addition of any osteogenic factors. We found that PPARγ‐siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARγ‐siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.