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Involvement of Rho/ROCK signalling in small cell lung cancer migration through human brain microvascular endothelial cells
Author(s) -
Li Bo,
Zhao Wei-Dong,
Tan Zhi-Min,
Fang Wen-Gang,
Zhu Li,
Chen Yu-Hua
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.056
Subject(s) - rhoa , rho associated protein kinase , microbiology and biotechnology , cell migration , phosphatidylinositol , kinase , actin cytoskeleton , focal adhesion , cytoskeleton , endothelial stem cell , protein kinase c , biology , pi3k/akt/mtor pathway , cancer research , cell , signal transduction , biochemistry , in vitro
Small cell lung cancer (SCLC) cells migration across human brain microvascular endothelial cells (HBMECs) is an essential step of brain metastases. Here we investigated signalling pathways in HBMECs contributing to the process. Inhibition of endothelial Rho kinase (ROCK) with Y27632 and overexpression of ROCK dominant‐negative mutant prevented SCLC cells, NCI‐H209, transendothelial migration and the concomitant changes of tight junction. Conversely, inhibition of phosphatidylinositol 3‐kinase (PI3K) and protein kinase C (PKC) had no effects. Furthermore, endothelial RhoA protein was activated during NCI‐H209 cells transendothelial migration. Rho/ROCK participated in NCI‐H209 cells transendothelial migration through regulating actin cytoskeleton reorganization. These results suggested that Rho/ROCK was required for SCLC cells transendothelial migration.